The PCR products were analyzed in 2% agarose gel electrophoresis. The amplification temperature was performed in 55✬.
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The presence of this toxin gene was verified through PCR and where were found in twenty-seven samples.
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The objective of this work was to investigate the presence of hpmA gene in forty-seven strains of uropathogenic P. mirabilis uropathogenesis HpmA hemolysin elicit tissue damage that allows entry of these bacteria into the kidney. Among these virulence factors there is the hemolysin of P. mirabilis expresses several virulence factors, such as urease, flagella, resistance to normal serum, IgG and IgA proteases, outer-membrane proteins and fimbriae. These infections may cause cystitis, pyelonephritis, bacteraemia and stone formation, which can cause severe damage in kidney tissue and may block catheters. Proteus mirabilis is a common cause of UTI in patients with short-and long-term indwelling urinary catheters, and in individuals with structural or functional abnormalities of the urinary tract. Patients of community and hospital may require catheterization for various reasons such as retention, acute or chronic urinary incontinence. The urinary catheter is one of the most commonly used medical procedures. Approximately 50% to 70% of women experience at least one episode of UTI during their lifetime, and 20% to 30% of recurrent episodes occur. Urinary tract infection (UTI) is characterized by invasion and bacterial multiplication in any follow-up urinary tract is a major query causes in medical practice, only getting behind respiratory infections.